ALL-TRANS-RETINOIC ACID AND HEXAMETHYLENE BISACETAMIDE (HMBA) REGULATE TGF-ALPHA AND HST-1/KFGF EXPRESSION IN DIFFERENTIATION SENSITIVE BUT NOT IN RESISTANT HUMAN TERATOCARCINOMAS

作者：MILLER WH; MAERZ WJ; KURIE J; MOY D; BASELGA J; LUCAS DA; GRIPPO JF; MASUI H; DMITROVSKY E

来源：Differentiation, 1994, 55(2): 145-152.

DOI：10.1046/j.1432-0436.1994.5520145.x

The multipotent human teratocarcinoma (TC) cell NTera-2 clone D1 (abbreviated NT2/D1) differentiates into a neuronal lineage after retinoic acid (RA) treatment and a distinct phenotype after hexamethylene bisacetamide (HMBA) treatment. We previously reported that RA treatment of NT2/D1 cells reduces cellular cloning efficiency and nude mouse tumorigenicity. This accompanied a loss of mRNA expression of transforming growth factor-alpha. (TGF-alpha) and the fibroblast growth factor kFGF, also known as hst-1 (abbreviated hst-1/kFGF). This study extends prior work by reporting that the distinct phenotype induced by HMBA also decreases cloning efficiency, tumorigenicity, and TGF-alpha and hst-1/kFGF mRNA expression in NT2/D1 cells. These RNA findings were confirmed by measurements of growth factor protein in the conditioned media of inducer-treated and untreated NT2/D1 cells. In two established human TC lines refractory to the actions of RA, N2102ep and Tera-1, RA fails to decrease expression of either growth factor despite induction of its nuclear receptor, RAR-beta. However, HMBA induces morphologic maturation and down-regulation of these growth factors in N2102ep cells. This indicates that the loss of TGF-alpha and hst-1/kFGF expression serves as a new marker of differentiation in human TCs. To explore the effects of these growth factors on growth and differentiation of NT2/D1 cells, TGF-alpha or hst-1/kFGF protein was added following inducer treatment or no treatment. Neither growth factor blocked immunophenotypic differentiation, but both promoted the growth of uninduced NT2/D1 cells in cloning assays. Growth factor expression studies were extended from cultured TCs to germ cell tumor biopsies, where TGF-alpha was expressed in all and hst-1/kFGF was expressed in a subset of examined tumors. Together, these findings reveal that TGF-alpha and hst-1/kFGF are differentiation markers in human teratocarcinomas that appear to promote tumor cell growth without blocking differentiation.

• 出版日期1994-1