Assessment of cytotoxicity and oxidative stress induced by titanium oxide nanoparticles on Chinook salmon cells

作者：Srikanth Koigoora^*; Pereira Eduarda; Duarte Armando C; Ahmad Iqbal; Rao Janapala Venkateswara

来源：Environmental Science and Pollution Research, 2015, 22(20): 15579-15586.

DOI：10.1007/s11356-015-4740-z

• Chinook salmon
• Titanium oxide nanoparticles
• Cytotoxicity
• Oxidative stress
• Lipid peroxidation

Titanium oxide nanoparticles (TiO2 NPs) have received wide attention in diverse application, but the potential impact of these nanomaterials on the environment, aquatic life and especially on fish cell lines is lacking. The present study aimed to investigate the cytotoxicity and oxidative stress induced by TiO2 NPs on Chinook salmon cells derived from Oncorhynchus tshawytscha embryos (CHSE-214). The The MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] and neutral red (NR) assays in CHSE-214 cells exposed to TiO2 NPs revealed concentration-dependent cytotoxic effect in the range of 10 to 60 mu g/ml for 24 h. CHSE-214 cells exposed to TiO2 NPs (10-60 mu g/ml) exhibited significant decline in superoxide dismutase (SOD), catalase (CAT) glutathione (GSH) content and increased lipid peroxidation (LPO) in a concentration-dependent manner. TiO2 NPs induced cytotoxicity and oxidative stress in CHSE-214 cells which serve as a base line studies for future studies.

• 出版日期2015-10