A TGF-beta receptor 1 inhibitor for prevention of proliferative vitreoretinopathy

作者：Nassar Khaled^*; Grisanti Swaantje; Tura Aysegul; Lueke Julia; Lueke Matthias; Soliman Mahmoud; Grisanti Salvatore

来源：Experimental Eye Research, 2014, 123: 72-86.

DOI：10.1016/j.exer.2014.04.006

• proliferative vitreoretinopathy
• penetrating eye injury
• vitrectomy
• TGF-beta receptor 1
• LY-364947

This study evaluates the use of the TGF-beta receptor I inhibitor LY-364947 (LY) to prevent proliferative vitreoretinopathy (PVR). For the in vitro experiments Human Tenon%26apos;s Fibroblasts (HTFs) and retinal pigment epithelial (RPE) cells were treated with different concentrations of LY to determine HTF proliferation and RPE transdifferentiation. For in vivo testing 30 rabbits underwent a PVR trauma model. The animals received different concentrations of intravitreally injected LY, with or without vitrectomy. LY treatment reduced HTF proliferation and RPE transdifferentiation in vitro. In vivo intravitreal injection of LY prevented PVR development significantly. This positive effect was also present when LY injection was combined with vitrectomy. Intravitreal injection of LY prevented tractional retinal detachment in 14 out of 15 animals. In conclusion, treatment with the TGF-beta receptor 1 inhibitor LY reduces HTF proliferation and RPE transdifferentiation in vitro and prevents proliferative vitreoretinopathy and subsequent tractional retinal detachment in vivo.

• 出版日期2014-6