A cationic fluorescent polymeric thermometer for the ratiometric sensing of intracellular temperature

作者：Uchiyama Seiichi^*; Tsuji Toshikazu; Ikado Kumiko; Yoshida Aruto; Kawamoto Kyoko; Hayashi Teruyuki; Inada Noriko

来源：Analyst, 2015, 140(13): 4498-4506.

DOI：10.1039/c5an00420a

We developed new cationic fluorescent polymeric thermometers containing both benzothiadiazole and BODIPY units as an environment-sensitive fluorophore and as a reference fluorophore, respectively. The temperature-dependent fluorescence spectra of the thermometers enabled us to perform highly sensitive and practical ratiometric temperature sensing inside living mammalian cells. Intracellular temperatures of non-adherent MOLT-4 (human acute lymphoblastic leukaemia) and adherent HEK293T (human embryonic kidney) cells could be monitored with high temperature resolutions (0.01-1.0 degrees C) using the new cationic fluorescent polymeric thermometer.

• 出版日期2015