Amino acids recognition by water-soluble uncharged porphyrin tweezers: Spectroscopic evidences in high optical density solutions

作者：Villari Valentina^*; Mineo Placido; Scamporrino Emilio; Micali Norberto

来源：Chemical Physics, 2012, 402: 118-123.

DOI：10.1016/j.chemphys.2012.04.018

• X-ray scattering
• Dysperse systems
• Supramolecular and biochemical assembly
• Biosensors

Small angle X-ray measurements on concentrated solutions of Cobalt-bis-porphyrins showed, at all the investigated concentration values, the presence of small aggregates which possess a sphere-like shape with a homogeneous electron density distribution. Such an aggregation, however, is proven not to affect the binding properties of the molecules with amino acids. Indeed, the Cobalt ion of the bis-porphyrins are available for coordinating the nitrogen atom of the amino acid to form a stable complex, as indicated by UV-vis and circular dichroism spectroscopy. The ability of these uncharged water-soluble bis-porphyrins to act as molecular sensors of amino acids in a wide concentration range takes great relevance in biosensing applications for which high concentration might be required.

• 出版日期2012-6-19