A single SNP, G929T (Gly310Val), determines the presence of a functional and a non-functional allele of HIS4 in Candida albicans SC5314: Detection of the non-functional allele in laboratory strains

作者：Gomez Raja Jonathan; Andaluz Encarnacion; Magee Beatrice; Calderone Richard; Larriba German^*

来源：Fungal Genetics and Biology, 2008, 45(4): 527-541.

DOI：10.1016/j.fgb.2007.08.008

• Candida albicans SC5314
• HIS4 heterozygosity
• SNPs

Candida albicans is a diploid organism that exhibits high levels of heterozygosity. Although the precise manner by which this heterozygosity provides advantage for the commensal/pathogenic life styles of C albicans is not known, heterozygous markers are themselves Useful for studying genomic rearrangements, which occur frequently in C albicans. Treatment of CAI-4 with UV light yielded histidine auxotrophs which could be complemented by HIS4, suggesting that strain CAI-4 is heterozygous for HIS4. These auxotrophs appeared to have undergone mitotic recombination and/or chromosome loss. As expected from a heterozygote, disruption of the functional allele of HIS4 resulted in a his4::hisG-URA3-hisG strain that is auxotrophic for histidine. Sequencing of random clones of the HIS4 ORF from CAI-4 and its precursor SC5314 revealed the presence of I I SNPs, seven synonymous and four non-synonymous. Site-directed mutagenesis indicates that only one of those SNPs, T929G (Gly310Val), is responsible for the non-functionality of the encoded enzyme. HIS4 analysis of five commonly used laboratory strains is reported. This study provides a new, easily measured nutritional marker that can be used in future genetic studies in C. albicans.

• 出版日期2008-4