Autophagy is required for CSF-1-induced macrophagic differentiation and acquisition of phagocytic functions

作者：Jacquel Arnaud; Obba Sandrine; Boyer Laurent; Dufies Maeva; Robert Guillaume; Gounon Pierre; Lemichez Emmanuel; Luciano Frederic; Solary Eric; Auberger Patrick^*

来源：Blood, 2012, 119(19): 4527-4531.

DOI：10.1182/blood-2011-11-392167

Autophagy is the process by which superfluous or damaged macromolecules or organelles are degraded by the lysosome. Pharmacologic and genetic evidence indicates that autophagy plays pleiotropic functions in cellular homeostasis, development, survival, and differentiation. The differentiation of human blood monocytes into macrophages is a caspase-dependent process when triggered ex vivo by colony stimulating factor-1. We show here, using pharmacologic inhibitors, siRNA approaches, and Atg7(-/-) mice, that autophagy initiated by ULK1 is required for proper colony stimulating factor-1-driven differentiation of human and murine monocytes. We also unravel a role for autophagy in macrophage acquisition of phagocytic functions. Collectively, these findings highlight an unexpected and essential role of autophagy during monocyte differentiation and acquisition of macrophage functions. (Blood. 2012; 119( 19): 4527-4531)

• 出版日期2012-5-10