Non-covalent modification of the active site of cytochrome P450 for inverting the stereoselectivity of monooxygenation

作者：Fujishiro Takashi; Shoji Osami; Watanabe Yoshihito^*

来源：Tetrahedron Letters, 2011, 52(3): 395-397.

DOI：10.1016/j.tetlet.2010.11.048

• Cytochrome P450
• Stereoselectivity
• Sulfoxidation
• Protein modification
• Hydrogen peroxide
• Biocatalyst

The enantioselectivity in the sulfoxidation of thioanisole catalyzed by cytochrome P450(BS beta) with a decoy molecule, a dummy molecule of the natural substrate, can be inverted by changing the structure of the decoy molecule. The methodology demonstrated herein shows the potential for controlling the stereoselectivity of biocatalysts without any mutagenesis.

• 出版日期2011-1-19