Ultra-weak reversible protein-protein interactions

作者：Rowe Arthur J^*

来源：Methods, 2011, 54(1): 157-166.

DOI：10.1016/j.ymeth.2011.02.006

• Analytical Ultracentrifugation
• Interactions
• NMR
• Protein-protein
• Ultra-weak

Ultra-weak interactions (K-d > 100 mu M) between proteins have in the last decade become an increasing focus of attention in cell biology, especially in relation to cell-cell interactions and signalling processes. Methods for their quantitative definition are reviewed. NMR spectroscopy plays a major role in this area, as it not only can define interactions as weak or weaker than 3 mM, but in favourable cases structural information concerning the complex can be yielded.
Free solution technologies mostly fail when addressed to such systems. The AUC has the highest practical capability, but evaluation of the data to yield K-a values is complicated by the presence of thermodynamic/hydrodynamic effects of a comparable order of magnitude. These effects can however be computationally removed by means of suitable algorithms, and K-d values of up to 50 mM can be characterised. The relative merits of velocity and equilibrium approaches are discussed, and both are shown to have particular advantages.

• 出版日期2011-5