A Molybdopterin Oxidoreductase Is Involved in H-2 Oxidation in Desulfovibrio desulfuricans G20

作者：Li Xiangzhen; Luo Qingwei; Wofford Neil Q; Keller Kimberly L; McInerney Michael J; Wall Judy D; Krumholz Lee R^*

来源：Journal of Bacteriology, 2009, 191(8): 2675-2682.

DOI：10.1128/JB.01814-08

Three mutants deficient in hydrogen/formate uptake were obtained through screening of a transposon mutant library containing 5,760 mutants of Desulfovibrio desulfuricans G20. Mutations were in the genes encoding the type I tetraheme cytochrome c(3) (cycA), Fe hydrogenase (hydB), and molybdopterin oxidoreductase (mopB). Mutations did not decrease the ability of cells to produce H-2 or formate during growth. Complementation of the cycA and mopB mutants with a plasmid carrying the intact cycA and/or mopB gene and the putative promoter from the parental strain allowed the recovery of H-2 uptake ability, showing that these specific genes are involved in H-2 oxidation. The mop operon encodes a periplasm-facing transmembrane protein complex which may shuttle electrons from periplasmic cytochrome c(3) to the menaquinone pool. Electrons can then be used for sulfate reduction in the cytoplasm.

• 出版日期2009-4-15