科研之友
微信
新浪微博
Facebook
分享链接
摘要
Two bacterial strains SZDIS-1-1(T) and GZDIS-1-1 were isolated from fermentation bed of a pigpen in Fujian Province, China. Cells were Gram-stain-positive, facultatively anaerobic, short rods without flagellum. Their nearest phylogenetic neighbours were Microbacterium amylolyticum N5(T) (16S rRNA gene sequence similarity 98.2%), Microbacterium indicum BBH6(T) (97.9%) and Microbacterium gubbeenense DPC 5286(T) (97.8%) with the DNA DNA hybridization values to strain SZDIS-1-1(T) as 20.0 +/- 1.2, 14.3 +/- 5.8 and 19.1 +/- 1.6%, respectively. The DNA G+C content of the new isolates was 67.6-71.1 mol% and anteiso-C-15:0, anteiso-C-17:0 and iso-C-16:0 were their predominant cellular fatty acids. These results were consistent with classification into the genus Microbacterium. However, cell-wall sugars and characteristic amino acid were rhamnose, glucose, galactose and ornithine. Major menaquinones were MK-11 and MK-10. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, glycolipids, unknown phospholipid and unknown lipids. These characteristics constructed a distinct profile of the two isolates. Therefore, based on polyphasic taxonomic characteristics, strains SZDIS-1-1(T) and GZDIS-1-1 represented a novel species of genus Microbacterium, for which the name Microbacterium sorbitolivorans sp. nov. is proposed, with strain SZDIS-1-1(T) (=CGMCC 1.15228(T)=DSM 103422(T)) as the type strain.
- 出版日期2016-12
- 单位贵州医科大学; 中国科学院微生物研究所
