Determination of Bisphosphonates by Ion-pair HPLC

作者：Matuszewski Lukasz; Matuszewska Anna; Mazurkiewicz Tomasz; Rogalski Jerzy; Cho Nam Seok; Ohga Shoji^*

来源：Journal of the Faculty of Agriculture, Kyushu University, 2011, 56(2): 213-216.

DOI：10.5109/20311

The optimization of the two bisphosphonates (disodium pamidronate and zoledronic acid) separation by ion-suppression and ion-pair high-performance liquid chromatography is described. The influence of the eluent pH, composition of the mobile phase and the concentration of complexing ion-pairing agents was investigated. This developed method provides high separation and specificity to bisphosphonate analysis. In quantitative analysis in the optimized conditions (10 mM n-amylamine or n-butylamine; 14% acetonitrile in 0.1 M sodium phosphate buffer, pH=7.0), the method showed good linearity (r=0.9999-0.9998) and sufficient sensitivity (10(-7)-10(-8)g/inl). It can be easily and conveniently adopted for the routine quality control analysis.

• 出版日期2011-9