Application of Isobaric Tags for Relative and Absolute Quantification (iTRAQ) Coupled with Two-Dimensional Liquid Chromatography/Tandem Mass Spectrometry in Quantitative Proteomic Analysis for Discovery of Serum Biomarkers for Idiopathic Pulmonary Fibrosis

作者：Zhang, Ying; Xin, Qian; Wu, Zhen; Wang, Chaochao; Wang, Yongbin; Wu, Qian; Niu, Rui^*

来源：MEDICAL SCIENCE MONITOR, 2018, 24: 4146-4153.

DOI：10.12659/MSM.908702

• Biological Markers
• Idiopathic Pulmonary Fibrosis
• Proteomics

Background: The present study was performed to explore the presence of informative protein biomarkers of human serum proteome in idiopathic pulmonary fibrosis (IPF).
Material/Methods: Serum samples were profiled using iTRAQ coupled with two-dimensional liquid chromatography/tandem mass spectrometry (2D-LC-MS/MS) technique, and ELISA was used to validate candidate biomarkers.
Results: A total of 394 proteins were identified and 97 proteins were associated with IPF. Four biomarker candidates generated from iTRAQ experiments - CRP, fibrinogen-alpha chain, haptoglobin, and kininogen-1 - were successfully verified using ELISA.
Conclusions: The present study demonstrates that levels of CRP and fibrinogen-alpha are higher and levels of haptoglobin and kininogen-1 are lower in patients with IPF compared to levels in healthy controls. We found they are useful candidate biomarkers for IPF.

• 出版日期2018-6-17
• 单位山东大学