ANKRD54 preferentially selects Bruton's Tyrosine Kinase (BTK) from a Human Src-Homology 3 (SH3) domain library

作者：Gustafsson Manuela O^*; Mohammad' Dara K; Ylosmaki Erkko; Choi Hyunseok; Shrestha Subhash; Wang Qing; Nore Beston F; Saksela Kalle; Smith C I Edvard^*

来源：PLos One, 2017, 12(4): e0174909.

DOI：10.1371/journal.pone.0174909

Bruton's Tyrosine Kinase (BTK) is a cytoplasmic protein tyrosine kinase with a fundamental role in B-lymphocyte development and activation. The nucleocytoplasmic shuttling of BTK is specifically modulated by the Ankyrin Repeat Domain 54 (ANKRD54) protein and the interaction is known to be exclusively SH3-dependent. To identify the spectrum of the ANKRD54 SH3-interactome, we applied phage-display screening of a library containing all the 296 human SH3 domains. The BTK-SH3 domain was the prime interactor. Quantitative western blotting analysis demonstrated the accuracy of the screening procedure. Revealing the spectrum and specificity of ANKRD54-interactome is a critical step toward functional analysis in cells and tissues.

• 出版日期2017-4-3