A microfluidics-based in situ chemotaxis assay to study the behaviour of aquatic microbial communities

作者：Lambert Bennett S; Raina Jean Baptiste; Fernandez Vicente I; Rinke Christian; Siboni Nachshon; Rubino Francesco; Hugenholtz Philip; Tyson Gene W; Seymour Justin R; Stocker Roman^*

来源：Nature Microbiology, 2017, 2(10): 1344-1349.

DOI：10.1038/s41564-017-0010-9

Microbial interactions influence the productivity and biogeochemistry of the ocean, yet they occur in miniscule volumes that cannot be sampled by traditional oceanographic techniques. To investigate the behaviours of marine microorganisms at spatially relevant scales, we engineered an in situ chemotaxis assay (ISCA) based on microfluidic technology. Here, we describe the fabrication, testing and first field results of the ISCA, demonstrating its value in accessing the microbial behaviours that shape marine ecosystems.

• 出版日期2017-10
• 单位MIT