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摘要
The detection of small molecules using aptamers is not straightforward when the induced fit triggered by the analyte is also small. Herein a labeling strategy is explored for the voltammetric detection of the antibiotic tobramycin using magnetic microparticles (MPs). A competition between MPs-immobilized tobramycin and free tobramycin for the limited amount of the biotinylated-aptamer was established. The addition of the enzymatic conjugate and the substrate generated a product that was electrochemically oxidized on screen-printed carbon electrodes. The analytical signal was proportional to the antibiotic concentration from 5-500 mu M and the reproducibility was 5.7%. The assay selectivity was also studied.
- 出版日期2011-1
