Development of fingerprinting for quality evaluation of Cyathula officinalis Kuan by LC-DAD-ESI-Q-TOF MS/MS coupled with multivariate statistical analysis

作者：Fan, Qiao-Jia; Liu, Jin-Liang; Sun, Lei; Zheng, Shun-Lin; Yuan, Ji-Chao^*; Tian, Meng-Liang; Kong, Fan-Lei

来源：Analytical Methods, 2015, 7(8): 3395-3401.

DOI：10.1039/c4ay02841d

• PERFORMANCE LIQUID-CHROMATOGRAPHY
• PHOTODIODE-ARRAY DETECTION
• RESPONSE-SURFACE METHODOLOGY
• QUANTITATIVE-ANALYSIS
• ASSISTED EXTRACTION
• HPLC FINGERPRINT
• GC-MS
• OPTIMIZATION
• ANGELICA
• RADIX

An LC-ESI-Q-TOF MS/MS method coupled with multivariate statistical analysis was developed for chemical fingerprinting of Cyathula officinalis Kuan. Ten peaks were selected as common peaks, and cyasterone was used as a reference. The relative areas of the common peaks were used for hierarchical clustering analysis, principal component analysis and similarity calculation. A total of 31 samples collected from different sources were classified into three groups (principal component analysis) or four groups (hierarchical clustering analysis). The similarities of the 31 batches of Cyathula officinalis samples were between 0.653 and 0.999. The results obtained suggested that the chromatographic fingerprint technique could efficiently evaluate C. officinalis.

• 出版日期2015
• 单位四川农业大学