dr1127: A novel gene of Deinococcus radiodurans responsible for oxidative stress

作者：Wu YuanYuan; Tian Bing; Hua YueJin^*

来源：Chinese Science Bulletin, 2007, 52(15): 2081-2087.

DOI：10.1007/s11434-007-0297-7

• dr1127
• Deinococcus radiodurans
• oxidative damage
• dsDNA binding

The functional analysis of dr1127, a novel gene in Deinococcus radiodurans was performed in this paper. The dr1127 gene was found occasionally in our microarray and 2-DE gel experiments. Mutation of the dr1127 gene decreased the gamma-radiation and H(2)O(2) resistance of D. radiodurans, and weakened the scavenging abilities of cell extracts for free radicals (superoxide anion, hydrogen peroxide, and hydroxyl radical). Further oxidative damage assays demonstrated that the purified DR1127 protein of D. radiodurans could bind to double stranded DNA in vitro and protect DNA from oxidative damage in this way. These results suggest that the dr1127 gene is an important gene that can maintain gamma-radiation and oxidative resistance in D. radiodurans and may take part in the oxidative stress process.

• 出版日期2007-8
• 单位浙江大学