ANALYSIS BY SOMATIC-CELL FUSION OF THE PHENOTYPIC PROPERTIES OF A MUTANT EMBRYONAL CARCINOMA CELL-LINE

作者：AZIZ N; DIETRICH J; SHIN HS; MCCORMICK PJ

来源：International Journal of Oncology, 1993, 3(2): 313-318.

• EMBRYONAL CARCINOMA
• RETROVIRAL INSERTIONAL MUTAGENESIS
• RA INDUCED DIFFERENTIATION
• TUMORIGENESIS

We have previously reported the isolation of an embryonal carcinoma (EC) cell mutant created via retroviral insertion (1). The mutant (NR1-6) is aberrant in respect to morphology, tumorigenicity and response to retinoic acid (RA). In order to determine the dominance/recessive hierarchy and genetic relationships of these phenotypes, we created hybrid lines from mutant and parental cells. Three hybrid clones (Hyb 1, 2a and 2b), which retain both mutant and parental loci, were analyzed. Our results indicate that in all parameters - morphology, adhesion, qualitative and quantitative response to RA and tumorigenicity - the hybrids behave similarly to parental cells. In no case did any of the hybrid lines exhibit a mutant phenotype. The data indicate that the phenotypes we have studied are genetically interrelated and that the parental characteristics are dominant.

• 出版日期1993-8