Analysis of antimicrobial peptides from porcine neutrophils

作者：Wessely Szponder Joanna^*; Majer Dziedzic Barbara; Smolira Anna

来源：Journal of Microbiological Methods, 2010, 83(1): 8-12.

DOI：10.1016/j.mimet.2010.07.010

• Antimicrobial peptides
• Cathelicidins
• Neutrophils
• Pig

Cationic host defence peptides are important components of innate immunity in pigs and other mammalians. Most of these peptides have a direct antimicrobial activity and they also have a broad spectrum of effects on the host immune system, which may be taken into account in the introduction of novel therapeutics. Our method permits simultaneous isolation of six antibacterial peptides, i.e. prophenin-1, prophenin-2, PR-39, and protegrins 1-3 from a porcine neutrophil crude extract and characterisation of them. Among the obtained peptides the greatest bactericidal activity expressed as MBC was seen in protegrins (10 mu g/ml), whereas in the other studied peptides MBC was on the level of 20 mu g/ml. Minimal inhibitory concentrations (MIC) reached 10 mu g/ml for protegrins 1-3 and 20 mu g/ml for prophenins, and PR-39. Within the bactericidal range all isolated peptides didn't show cytotoxicity on cell lines used in our experiment.

• 出版日期2010-10