Antibody fragment-conjugated polymeric micelles incorporating platinum drugs for targeted therapy of pancreatic cancer

作者：Ahn Jooyeon; Miura Yutaka; Yamada Naoki; Chida Tsukasa; Liu Xueying; Kim Ahram; Sato Ryuta; Tsumura Ryo; Koga Yoshikatsu; Yasunaga Masahiro; Nishiyama Nobuhiro; Matsumura Yasuhiro; Cabral Horacio; Kataoka Kazunori^*

来源：Biomaterials, 2015, 39: 23-30.

DOI：10.1016/j.biomaterials.2014.10.069

• Drug delivery
• Platinum
• Micelle
• Nanoparticle
• Chemotherapy

Antibody-mediated therapies including antibody-drug conjugates (ADCs) have shown much potential in cancer treatment by tumor-targeted delivery of cytotoxic drugs. However, there is a limitation of pay-loads that can be delivered by ADCs. Integration of antibodies to drug-loaded nanocarriers broadens the applicability of antibodies to a wide range of therapeutics. Herein, we developed antibody fragment-installed polymeric micelles via maleimide-thiol conjugation for selectively delivering platinum drugs to pancreatic tumors. By tailoring the surface density of maleimide on the micelles, one tissue factor (TF)-targeting Fab' was conjugated to each carrier. Fab'-installed platinum-loaded micelles exhibited more than 15-fold increased cellular binding within 1 h and rapid cellular internalization compared to non-targeted micelles, leading to superior in vitro cytotoxicity. In vivo, Fab'-installed micelles significantly suppressed the growth of pancreatic tumor xenografts for more than 40 days, outperforming non-targeted micelles and free drugs. These results indicate the potential of Fab'-installed polymeric micelles for efficient drug delivery to solid tumors.

• 出版日期2015-1