科研之友
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摘要
The promoter region of the Acinetobacter calcoaceticus encoded trpFB operon was determined and found to contain dual promoters. The gene expression of trpFB operon was studied as a function of different concentrations of tryptophan and general amino acid starvation. The amounts of mRNA were determined by primer extensions and the expression of transcriptional and translational lacZ fusions was studied. The change in the concentration of tryptophan had no effect on transcription or translation, however, a general metabolic effect of increased transcription upon the starvation of all amino acids was detected. It is concluded that trpFB is constitutively expressed and lacks regulation.
- 出版日期1998-4-25
