Mitochondrial Respiration in the Platelets of Patients with Alzheimer's Disease

作者：Fisar Zdenek^*; Hroudova Jana; Hansikova Hana; Spacilova Jana; Lelkova Petra; Wenchich Laszlo; Jirak Roman; Zverova Martina; Zeman Jiri; Martasek Pavel; Raboch Jiri

来源：Current Alzheimer Research, 2016, 13(8): 930-941.

DOI：10.2174/1567205013666160314150856

• Alzheimer's disease
• biomarker
• mitochondrion
• platelet
• respiratory chain
• respiratory rate

Mitochondrial dysfunctions significantly contribute to the pathogenesis of Alzheimer's disease (AD). Here, we studied the relationship between AD and changes in the mitochondrial rates of respiration in blood platelets, respiratory chain complexes activity, and coenzyme Q(10) plasma concentrations. In intact platelets obtained from AD patients, we observed a decrease in endogenous basal respiration rates, a decrease in the maximal capacity of the electron transport system (ETS), and higher respiratory rates after inhibiting complex I of the ETS. When normalized for citrate synthase activity, rotenone inhibited respiratory rates and complex I activity was significantly altered. In permeabilized platelets, mitochondrial respiration was completely rescued by the addition of complex I substrates. The changes in mitochondrial respiratory parameters were not associated with the progression of AD except for the capacity of the ETS in permeabilized platelets. In AD, complex I activity was increased, complex IV activity was decreased, and coenzyme Q(10) plasma concentrations were decreased. Our data indicate that both insufficiency in substrates entering into the oxidative phosphorylation system and functional disturbances in the ETS complex are responsible for the decrease in respiration observed in intact platelets in AD patients. Analyses of complex IV activity, the respiratory rates of intact platelets, and the capacity of the ETS in permeabilized platelets may enable the characterization of mitochondrial dysfunctions in the initial stage of AD.

• 出版日期2016