NUCLEOTIDE-SEQUENCE OF NIFD FROM FRANKIA-ALNI STRAIN ARI3 - PHYLOGENETIC INFERENCES

作者：NORMAND P^*; GOUY M; COURNOYER B; SIMONET R

来源：Molecular Biology and Evolution, 1992, 9(3): 495-506.

DOI：10.1093/oxfordjournals.molbev.a040737

• NIFD
• FRANKIA-ALNI
• MOLECULAR PHYLOGENY

The complete nucleotide sequence of the nifD gene encoding the a subunit of component I of nitrogenase from Frankia alni strain ArI3 was determined. The coding region is 1,458 bp in length and encodes a polypeptide of 486 residues with a predicted molecular weight of 53,500. Phylogenetic inferences with 12 complete published nifD sequences were drawn using a variety of approaches. Frankia nifD clusters with proteobacteria rather than with Clostridium pasteurianum, the other Gram-positive bacterium studied. Extant eubacterial nif genes seem to have at least three distinct evolutionary origins as a result of ancien t gene duplications. Within the Gram-positive bacterial phylum, functional nif genes descend from different duplicates.

• 出版日期1992-5