An improved water-soluble/stereospecific biotransformation of aporphine alkaloids in Stephania epigaea to 4R-hydroxyaporphine alkaloids by Clonostachys rogersoniana

作者：Cai, Le; Dong, Jian-Wei; Zhao, Li-Xing; Zhou, Hao; Xing, Yun; Li, Ying; Li, Zhen-Jie; Duan, Wei-He; Li, Xue-Jiao; Ding, Zhong-Tao^*

来源：Process Biochemistry, 2016, 51(8): 933-940.

DOI：10.1016/j.procbio.2016.04.016

• Aporphine alkaloid
• 4R-Hydroxyaporphine alkaloid
• Stephania epigaea
• Biotransformation
• Clonostachys rogersoniana
• Water solubility

Aporphine alkaloids were transformed into the corresponding stereospecific 4R-hydroxyaporphine alkaloids through the solid-state fermentation of Stephania epigaea with Clonostachys rogersoniana. This process was validated by both solid- and liquid-state fermentations with aporphine alkaloids as substrates. Cytochrome P450 enzymes were confirmed to participate in the catalysis of this biotransformation. 4R-Hydroxyaporphine alkaloids exhibit the same levels of acetylcholinesterase (AChE) inhibitory and cytotoxic activities as aporphine alkaloids and are considerably more water soluble than aporphine alkaloids, indicating their potential as water-soluble AChE inhibitors and antitumor agents. This paper suggests that C. rogersoniana fermentation can facilitate a novel biotransformation of aporphine alkaloids in S. epigaea to 4R-hydroxyaporphine alkaloids.

• 出版日期2016-8
• 单位云南大学