Analysis of second-harmonic-generation microscopy in a mouse model of ovarian carcinoma

作者：Watson Jennifer M; Rice Photini F; Marion Samuel L; Brewer Molly A; Davis John R; Rodriguez Jeffrey J; Utzinger Urs; Hoyer Patricia B; Barton Jennifer K^*

来源：Journal of Biomedical Optics, 2012, 17(7): 076002.

DOI：10.1117/1.JBO.17.7.076002

• collagen
• ovarian cancer
• multiphoton
• two-photon
• imaging
• 7,12-dimethylbenz[a]anthracene

Second-harmonic-generation (SHG) imaging of mouse ovaries ex vivo was used to detect collagen structure changes accompanying ovarian cancer development. Dosing with 4-vinylcyclohexene diepoxide and 7,12-dimethylbenz[a]anthracene resulted in histologically confirmed cases of normal, benign abnormality, dysplasia, and carcinoma. Parameters for each SHG image were calculated using the Fourier transform matrix and gray-level co-occurrence matrix (GLCM). Cancer versus normal and cancer versus all other diagnoses showed the greatest separation using the parameters derived from power in the highest-frequency region and GLCM energy. Mixed effects models showed that these parameters were significantly different between cancer and normal (P %26lt; 0.008). Images were classified with a support vector machine, using 25% of the data for training and 75% for testing. Utilizing all images with signal greater than the noise level, cancer versus not-cancer specimens were classified with 81.2% sensitivity and 80.0% specificity, and cancer versus normal specimens were classified with 77.8% sensitivity and 79.3% specificity. Utilizing only images with greater than of 75% of the field of view containing signal improved sensitivity and specificity for cancer versus normal to 81.5% and 81.1%. These results suggest that using SHG to visualize collagen structure in ovaries could help with early cancer detection.

• 出版日期2012-7