Applicability of the TCDD-TEQ approach to predict sublethal embryotoxicity in Fundulus heteroclitus

作者：Rigaud Cyril^*; Couillard Catherine M; Pellerin Jocelyne; Legare Benoit; Hodson Peter V

来源：Aquatic Toxicology, 2014, 149: 133-144.

DOI：10.1016/j.aquatox.2014.02.002

• Fundulus heteroclitus
• TEF
• PCB
• Furan
• Aroclor 1254
• Behavior

The 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) toxic equivalent quantity (TCDD-TEQ) approach was used successfully to predict lethal embryotoxicity in salmonids, but its applicability to sublethal effects of mixtures of organohalogenated compounds in other fish species is poorly known. The sublethal toxicity of two dioxin-like compounds (DLCs), 3,3 ',4,4 '-tetrachlorobiphenyl (PCB77) and 2,3,4,7,8-pentachlorodibenzofuran (2,3,4,7,8-PnCDF), two non-dioxin-like (NDL) polychlorinated biphenyls (PCBs), 2,2 ',5,5 '-tetrachlorobiphenyl (PCB52) and 2,3,3 ',4 ',6-pentachlorobiphenyl (PCB110), and of Aroclor 1254, a complex commercial mixture of PCBs, was assessed in Fundulus heteroclitus embryos exposed by intravitelline injection. At 16 days post-fertilization, the two DLCs and Aroclor 1254 altered prey capture ability in addition to inducing classical aryl hydrocarbon receptor-mediated responses: ethoxyresorufin-O-deethylase (EROD) induction, craniofacial deformities and reduction in body length. None of these responses was induced by the two NDL PCBs, at doses up to 5400 ngg(-1) wet weight. Dose-response curves for prey capture ability for the 2 DLCs tested were not parallel to that of TCDD, violating a fundamental assumption for relative potency (ReP) estimation. Dose-response curves for EROD induction were parallel for 2,3,4,7,8-PnCDF and TCDD, but the ReP of 2,3,4,7,8-PnCDF for F. heteroclitus was 5-fold higher than the World Health Organization (WHO) fish toxic equivalent factor (TEF) based on embryolethality in salmonids. The chemically derived TCDD-TEQs of Aroclor 1256 3,3 ',4,4 ',5-pentachlorobiphenyl (PCB126) concentrations and it ReP far potency to induce EROD activity possibly due to antagonistic interactibaslights the limitations of using TEFs based on salmonid toxicity data alone for risk assess fish species. There is a need to assess the variability of RePs of DLCs in different species for a variety of endpoints and to better understand interactions between DLCs and other toxic chemicals.

• 出版日期2014-4