Mast Cells Boost Myeloid-Derived Suppressor Cell Activity and Contribute to the Development of Tumor-Favoring Microenvironment

作者：Danelli Luca; Frossi Barbara; Gri Giorgia; Mion Francesca; Guarnotta Carla; Bongiovanni Lucia; Tripodo Claudio; Mariuzzi Laura; Marzinotto Stefania; Rigoni Alice; Blank Ulrich; Colombo Mario P; Pucillo Carlo E^*

来源：Cancer Immunology Research, 2015, 3(1): 85-95.

DOI：10.1158/2326-6066.CIR-14-0102

Inflammation plays crucial roles at different stages of tumor development and may lead to the failure of immune surveillance and immunotherapy. Myeloid-derived suppressor cells (MDSC) are one of the major components of the immune-suppressive network that favors tumor growth, and their interaction with mast cells is emerging as critical for the outcome of the tumor-associated immune response. Herein, we showed the occurrence of cell-to-cell interactions between MDSCs and mast cells in the mucosa of patients with colon carcinoma and in the colon and spleen of tumor-bearing mice. Furthermore, we demonstrated that the CT-26 colon cancer cells induced the accumulation of CD11b(+)Gr1(+) immature MDSCs and the recruitment of protumoral mast cells at the tumor site. Using ex vivo analyses, we showed that mast cells have the ability to increase the suppressive properties of spleen-derived monocytic MDSCs, through a mechanism involving IFN gamma and nitric oxide production. In addition, we demonstrated that the CD40:CD40L cross-talk between the two cell populations is responsible for the instauration of a proinflammatory microenvironment and for the increase in the production of mediators that can further support MDSC mobilization and tumor growth. In light of these results, interfering with the MDSC: mast cell axis could be a promising approach to abrogate MDSC-related immune suppression and to improve the antitumor immune response.

• 出版日期2015-1