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摘要
The aim of this work was to obtain well-defined HyPG-MA (methacrylated hyperbranched polyglycerol) microparlicles with uniform sizes. Therefore, three different preparation methods were evaluated. First, we assessed a micromolding technique using rigid SU-8 (a photoresist based on epoxies) grids. Independent of the surface treatment of the SU-8 grid or the type of polymer used, approximately 50% of the microgels remained attached to the SU-8 grid or broke into smaller particles during the release process in which drying of the gels was followed by a sonication process. Although 90% methacrylate conversion could be obtained, this method has some additional drawbacks as the obtained dried microgels did not rehydrate completely after the drying step. Second, a soft micromolding technique was evaluated using elastomeric PDMS (poly(dimethyl siloxane)) grids. The use of these flexible grids resulted in a high yield (80-90% yield; > 90% methacrylate conversion) of microgels with a well-defined size and shape (squares 100 mu m x 100 mu m x 50 mu m or hexagons with phi 30 mu m and a thickness of 20 mu m) without the occurrence of water evaporation. However, a number of particles showed a less-defined shape as not all grids could be filled well. The microgets showed restricted swelling, implying that these gels are dimensionally stable. Third, an alternative method referred to as photolithography was evaluated. This method was suitable to tailor accurately the size and shape of HyPG-MA microgels and additionally gained 100% yield. Well-defined HyPG-MA microgels in the size range of 200-1400,mu m (thickness of 6, 20, or 50,mu m), with a methacrylate conversion of > 90%, could easily be prepared by adding an inhibitor (e.g., 1% (w/v) of vitamin Q to the polymer solution to inhibit dark polymerization. Microgels in the size range of 30-100 mu m (> 90% conversion) could only be obtained when applying the photomask in direct contact with the polymer solution and using a higher (i.e., 2% (w/v)) concentration of vitamin C. Additionally, the microgels showed limited swelling, indicating that rather dimensionally stable particles were obtained. In conclusion, this paper shows that photolithography and soft micromolding, as compared to rigid micromolding, are the most appropriate techniques to fabricate structured HyPG-MA microgels with a tailorable and well-defined size and shape. These microgels have great potential in tissue engineering and drug delivery applications.
- 出版日期2007-11-6
