科研之友
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摘要
Gametophytic self-incompatibility (SI), an important character for breeding seedless cultivars of Citrus, is known in pummelo, mandarin, and many hybrid cultivars with pummelo in their pedigrees. Only a little is known about the allelic variation in the self-incompatibility gene (S),S allele frequencies, and the genotypes of self-incompatible and semi-self-compatible cultivars. In this study, pollination of `Banpeiyu' S-1 seedlings with `Banpeiyu' and pollination between the SI seedlings were performed to determine homozygous Si seedlings for the S gene. Seventy-eight Citrus accessions, including 55 pummelo accessions, were pollinated with each of two homozygous S-1 seedlings (S1S1 and S2S2). Pollen tube arrest in the style base of their pollinated pistils indicated that 23 accessions, including `Banpeiyu', have an S-1 allele each and 16 accessions, including 'Banpeiyu', have an S-2 allele each. Frequency of accessions with Si allele was 29.9% (23 of 77 accessions examined) and Si allele frequency was 16.4% (23 of 140 alleles excluding S-f allele). Frequency of accessions with S-2 allele was 21.3% (16 of 75 accessions examined) and S-2 allele frequency was 11.6% (16 of 138 alleles excluding Sf allele). Pummel() accessions collected from Kagoshima Prefecture had Si alleles with two and half times higher frequency (56.3%) than that in all accessions examined. Of the 79 accessions, six accessions ('Banpeiyu', 'Iriki Buntan', 'Kaopang', Nagashima Buntan No. 6, Nagashima Buntan No. 7, and `Soyu') were S1S2 genotypes. The Citrus cultivars, whose S genotypes have been fully determined in this study, were 'Banpeiyu' (S1S2), Iriki Buntan' (S1S2), 'Kaopang' (S1S2), `Soyu' (S1S2), 'Kinukawa' (SfS2), and 'Kawano Natsudaidai' (SfS2).
- 出版日期2011-10
