Attenuation of autophagic-proteolysis in C2C12 cells by saccharopine

作者：Sato Tomonori; Ito Yoshiaki^*; Nagasawa Takashi

来源：Molecular and Cellular Biochemistry, 2015, 410(1-2): 93-100.

DOI：10.1007/s11010-015-2541-9

• Lysine
• Saccharopine
• Autophagy
• mTOR
• Akt
• Sarcopenia

Muscle wasting impairs physical function and leads people to a bedridden state. We previously demonstrated that lysine (Lys) suppresses autophagic-proteolysis through the Akt pathway. However, the effect of metabolites of Lys on proteolysis is unclear. In this study, we investigated the effect of saccharopine (Sac), a metabolite of Lys, on proteolysis in C2C12 cells. When C2C12 myotubes were incubated in serum-free medium containing Sac, the rate of proteolysis, which was evaluated by 3-methylhistidine released from C2C12 myotubes, and autophagy activity, which was assessed by amount of light chain 3-II, were suppressed. Sac stimulated Akt and mammalian target of rapamycin signaling, which was evaluated from eIF4E-binding protein 1 phosphorylation. The suppressive effects of Sac on proteolysis and autophagy were completely abolished by an Akt inhibitor. Therefore, we concluded that Sac suppresses autophagic-proteolysis through Akt as with Lys.

• 出版日期2015-12