Anti-Acanthamoeba Activity of Multi-purpose Contact Lens Disinfecting Solutions against Acanthamoeba Cysts of Clinical and Environmental Isolates

作者：Abd Ghani Mohamed Kamel^*; Omar Syamimi; Nordin Anisah; Suboh Yusof; Ab Rahim Noraina; Abdullah Wan Omar; Ahmad Norazah

来源：Sains Malaysiana, 2016, 45(4): 609-613.

• Acanthamoeba
• effectiveness
• Malaysia
• multi-purpose contact lens disinfecting solution

Effective disinfection of contact lenses is of paramount importance in the prevention of Acanthamoeba (AK). Therefore, this study was undertaken to evaluate the effectiveness of the multi-purpose contact lens disinfecting solutions against Acanthamoeba cysts of four isolates comprising two clinical isolates; HS 5, HTH 73, and two environmental isolates; TTA 1 and TKA 14. Five multi-purpose contact lens disinfecting solutions; Complete (R), Renu (R) fresh (TM), RevitaLens OcuTec (R), Opti-Free (R) Express (R) and Solo Care Aqua (R) were tested on cyst of the four Acanthamoeba isolates. The soaking times were based on manufactures recommendations (4 and 6 h), 8 h and 24 h. Each mixture of the cysts and disinfecting solution was transferred onto non-nutrient agar seeded with Escherichia coli. The agar plates were examined under inverted microscope daily until day 14 to detect the presence of Acanthamoeba trophozoites. All 5 multi-purpose contact lens disinfecting solutions tested were ineffective at killing all Acanthamoeba cyst isolates tested. This indicates that most multi-purpose contact lens disinfecting solutions do not have anti-Acanthamoeba activity necessary to prevent keratitis amongst the contact lens wearers.

• 出版日期2016-4