Toll-like receptor 5 (TLR5), IL-1 beta secretion, and asparagine endopeptidase are critical factors for alveolar macrophage phagocytosis and bacterial killing

作者：Descamps Delphyne; Le Gars Mathieu; Balloy Viviane; Barbier Diane; Maschalidi Sophia; Tohme Mira; Chignard Michel; Ramphal Reuben; Manoury Benedicte^*; Sallenave Jean Michel

来源：Proceedings of the National Academy of Sciences of the United States of America, 2012, 109(5): 1619-1624.

DOI：10.1073/pnas.1108464109

• flagellin
• interleukin-1
• lysosomal protease

A deficit in early clearance of Pseudomonas aeruginosa (P. aeruginosa) is crucial in nosocomial pneumonia and in chronic lung infections. Few studies have addressed the role of Toll-like receptors (TLRs), which are early pathogen associated molecular pattern receptors, in pathogen uptake and clearance by alveolar macrophages (AMs). Here, we report that TLR5 engagement is crucial for bacterial clearance by AMs in vitro and in vivo because unflagellated P. aeruginosa or different mutants defective in TLR5 activation were resistant to AM phagocytosis and killing. In addition, the clearance of PAK (a wild-type P. aeruginosa strain) by primary AMs was causally associated with increased IL-1 beta release, which was dramatically reduced with PAK mutants or in WT PAK-infected primary TLR5(-/-) AMs, demonstrating the dependence of IL-1 beta production on TLR5. We showed that this IL-1 beta production was important in endosomal pH acidification and in inducing the killing of bacteria by AMs through asparagine endopeptidase (AEP), a key endosomal cysteine protease. In agreement, AMs from IL-1R1(-/-) and AEP(-/-) mice were unable to kill P. aeruginosa. Altogether, these findings demonstrate that TLR5 engagement plays a major role in P. aeruginosa internalization and in triggering IL-1 beta formation.

• 出版日期2012-1-31