摘要
background: the diagnosis of lymphoproliferative disorders (lpd) is routinely made through histological and immunohistochemical analysis of lymph nodes. immunophenotyping by flow cytometry (fc) is a sensitive and fast tool, which may be applied in samples obtained through fine needle aspiration for the diagnosis of lpd. bcl-2 is a proto-oncogene that appears in several lpd and it has a significantly high expression in follicular lymphomas. objectives: to diagnose lpd in fna samples through morphology and flow cytometry immunophenotyping. material and methods: samples from 25 patients with lymphadenopathies and 2 reactive tonsils were studied through morphology and immunophenotyping. the antigens expressions were evaluated by using a screening panel of monoclonal antibodies (cd3, cd4, cd8, cd19, light chains kappa; and lambda), followed by cd5, cd10, cd11c, cd23, cd79b, sigm, fmc-7 and bcl-2 when required. the results were compared with histology. results:four out of 25 samples were reactive processes and 21were b-lpd. in all cases there was consistency with histological results. the mean fluorescence intensity of bcl-2 in follicular lymphoma (19.92) was higher compared with other lymphoproliferative diseases (11.93) and controls (3.49) (p = 0.032). conclusion: fine needle aspiration of lymph nodes associated with cytomorphology and flow cytometry immunophenotyping allows a fast differentiation between reactive processes and b lymphoproliferative cases. the high expression of bcl-2 by cytometry shows its usefulness in the diagnosis of the most frequent type of b-lpd. fine needle aspiration sampling requires training and more than one aspiration is recommended.
- 出版日期2008