科研之友
微信
新浪微博
Facebook
分享链接
摘要
According to the principle of the conductivity change of the whole network caused by the variation of its bandgap and the contact resistance between tubes, carbon nanotube was used to develop a highly sensitive paper sensor for the detection of alpha-fetoprotein (AFP). Carbon nanotubes were functionalized by F108 to prepare uniform, stable dispersion where anti. AFP was further added in. Filter paper was dipped into the suspension in order to make it conductive. The rapid, accurate AFP detection could be accomplished by measuring the conductivity change of the paper sensor caused by immune reaction between immobilized antibody on the paper and AFP. This paper sensor with 12 dip. coats exhibited sensitive linear responses to AFP in the concentration range from 0.156 ng/mL to 100 ng/mL with a limit of detection of 0.156 ng/mL. With its simplicity, low cost, high sensitivity and accuracy, the constructed paper sensor based on carbon nanotube could detect AFP concentration within 5 min with good repeatability. The detection results of AFP in real serum samples by the proposed sensor were consistent with those by traditional ELISA method.
- 出版日期2015-10
- 单位西北农林科技大学
