Adsorption of bovine serum albumin on template-polymerized chitosan/poly(methacrylic acid) complexes

作者：de Vasconcelos C L; Bezerril P M; Dantas T N C; Pereira M R; Fonseca J L C^*

来源：Langmuir, 2007, 23(14): 7687-7694.

DOI：10.1021/la700537t

Particulate systems composed of polyelectrolyte complexes (PEC) based on chitosan and poly(methacrylic acid) were obtained via template polymerization. The resultant particles were characterized as having regions with different charge densities: chitosan predominating in the core and poly(methacrylic acid) at the surface, the particles being negatively charged, as a consequence. Albumin was adsorbed on these particles (after cross-linking with glutardialdehyde), and pH was controlled to obtain two conditions: (i) adsorption of positively charged albumin and (ii) adsorption of albumin at its isoelectric point. Adsorption isotherms and zeta-potential measurements showed that albumin adsorption was controlled by hydrogen bonding/ van der Waals interactions and that brush-like structures may enhance the adsorption of albumin on these particles. It was also found that shearing can induce desorption of albumin from the PEC surface, depending on the continuous phase albumin concentration.

• 出版日期2007-7-3