Ultrasensitive electrochemical DNA detection based on dual amplification of circular strand-displacement polymerase reaction and hybridization chain reaction

作者：Wang, Cui; Zhou, Hui; Zhu, Wenping; Li, Hongbo; Jiang, Jianhui^*; Shen, Guoli; Yu, Ruqin

来源：Biosensors and Bioelectronics, 2013, 47: 324-328.

DOI：10.1016/j.bios.2013.03.020

• Circular strand-displacement polymerase reaction
• Hybridization chain reaction
• Electrochemical DNA sensor
• Signal amplification

We developed a novel electrochemical strategy for ultrasensitive DNA detection using a dual amplification strategy based on the circular strand-displacement polymerase reaction (CSDPR) and the hybridization chain reaction (HCR). In this assay, hybridization of hairpin-shaped capture DNA to target DNA resulted in a conformational change of the capture DNA with a concomitant exposure of its stem. The primer was then hybridized with the exposed stem and triggered a polymerization reaction, allowing a cyclic reaction comprising release of target DNA, hybridization of target with remaining capture DNA, polymerization initiated by the primer. Furthermore, the free part of the primer propagated a chain reaction of hybridization events between two DNA hairpin probes with biotin labels, enabling an electrochemical reading using the streptavidin-alkaline phosphatase. The proposed biosensor showed to have very high sensitivity and selectivity with a dynamic response range through 10 fM to 1 nM, and the detect limit was as low as 8 fM. The proposed strategy could have the potential for molecular diagnostics in complex biological systems.

• 出版日期2013-9-15
• 单位湖南大学