Determination of telbivudine in the plasma of chronic hepatitis B patients in long-term treatment by high-performance liquid chromatographic-tandem mass spectrometry

作者：Chen, Bicui; Chen, Li; Cheng, Cai; Zhong, Mingkang; Shi, Xiaojin; Zhang, Jiming^*; Wang, Bin^*

来源：Biomedical Chromatography, 2018, 32(4): e4140.

DOI：10.1002/bmc.4140

• creatine kinase
• mitochondrial toxicity
• myopathy
• pharmacokinetics
• quantitation
• telbivudine

Creatine kinase elevation is commonly reported in telbivudine-treated patients. However, little is known about the relationship between this adverse drug reaction and plasma concentration. In this study, a sensitive, rapid and safe quantitative bioanalytical method has been established by using LC-MS/MS for the determination of telbivudine in a clinical study of chronic hepatitis B patients. The assay was linear in a dynamic 10-10,000 ng/mL range (r(2)> 0.999) and total analysis time was 6 min in this method. The validated method was applied to quantitatively determine plasma concentration in chronic hepatitis B patients during long-term telbivudine treatment. The results revealed that telbivudine concentration in the creatine kinase-elevated group (707.92-2788.78 ng/mL) was significantly higher than those with normal creatine kinase (412.63-1108.32 ng/mL). This method was adapted for therapeutic drug monitoring.

• 出版日期2018-4
• 单位复旦大学