A simple and reliable method to conduct and monitor expression cassette integration into the Escherichia coli chromosome

作者：Albermann Christoph^*; Trachtmann Natalie; Sprenger Georg A

来源：Biotechnology Journal, 2010, 5(1): 32-38.

DOI：10.1002/biot.200900193

• Chromosomal integration
• Escherichia coli
• MacConkey screening
• Zeaxanthin

We report a method for the integration of expression cassettes into the Escherichia coli chromosome using rare and dispensable sugar degradation gene loci as sites for integration. Clones carrying successfully recombined DNA fragments in the chromosome are easily screened using a solid differential medium containing the respective sugar compound. As an example for the heterologous expression of a complex natural product biosynthesis pathway, we show the step-wise chromosomal integration of the zeaxanthin biosynthesis pathway from Pontoea ananatis into E. coli.

• 出版日期2010-1