Association Between High-Mobility Group Box-1 Protein Release and Immune Function of Dendritic Cells in Thermal Injury

作者：Zhang, Li-Tian; Yao, Yong-Ming^*; Yao, Feng-Hua; Huang, Li-Feng; Dong, Ning; Yu, Yan; Sheng, Zhi-Yong

来源：Journal of Interferon and Cytokine Research, 2010, 30(7): 487-495.

DOI：10.1089/jir.2009.0055

• GLYCATION END-PRODUCTS
• BURN INJURY
• NEURITE OUTGROWTH
• DOWN-REGULATION
• NECROTIC CELLS
• SEVERE SEPSIS
• IFN-GAMMA
• T-CELLS
• HMGB1
• ACTIVATION

The present study was performed to investigate in vivo the effect of high-mobility group box-1 protein (HMGB1) on the maturation of dendritic cell (DC) and the influence on T-cell-mediated immunity after thermal injury. Rats were randomly divided into 3 groups as follows: sham burn group, burn group, and burn with ethyl pyruvate (EP) treatment group, and they were sacrificed on post burn days (PBD) 1, 3, 5, and 7 respectively. MACS micro-beads were used to isolate splenic DCs and column of nylon wool to obtain T cells. Phenotypes were analyzed by flow cytometry and cytokines were determined with ELISA kits. The expression levels of splenic HMGB1 were significantly elevated during PBD 1-7. DC expressed similar CD80 levels, strongly enhanced CD86, and slightly elevated MHC class II levels in comparison to DC from sham-injured rats, and protein levels of IL-12 were not increased after thermal injury. Administration of EP to inhibit HMGB1 could significantly enhance expression levels of CD80, MHC class II on DC surface, and IL-12 production after burns. Simultaneously, proliferative activity and expression levels of IL-2 as well as IL-2R alpha of T cell were restored. These results suggested that the excessively released HMGB1 might stimulate splenic DC to mature abnormally and down-regulate the IL-12 production, and further shifting of Th1 to Th2 with suppression of T-lymphocyte immune function following burn injury.

• 出版日期2010-7
• 单位中国人民解放军总医院