Amino acid signatures in the Ovar-DRB1 peptide-binding pockets are associated with Ovine Pulmonary Adenocarcinoma susceptibility/resistance

作者：Larruskain Amaia^*; Minguijon Esmeralda; Garcia Etxebarria Koldo; Arostegui Inmaculada; Moreno Bernardino; Juste Ramon A; Jugo Begona M

来源：Biochemical and Biophysical Research Communications, 2012, 428(4): 463-468.

DOI：10.1016/j.bbrc.2012.10.085

• Ovine Pulmonary Adenocarcinoma
• Ovine major histocompatibility complex
• Ovar-DRB1
• Amino acid motif
• Association analysis
• PCR-SBT

Potential relationships between amino acid motifs of various alleles of the ovine major histocompatibility complex DR (Ovar-DR) molecule and occurrence of clinical OPA caused by JSRV were investigated in a case-control study. Latxa sheep (n = 132) screened for presence/absence of pulmonary OPA lesions were typed for their Ovar-DRB1 2nd exon alleles by PCR and sequence-based typing (PCR-SBT). The polymorphic amino acid residues derived from the obtained 34 DRB1 protein variants were subjected to a logistic regression-based association study. The amino acids at several positions showed significant associations with the presence/absence of pulmonary OPA lesions: some of the residues were located within the peptide binding cleft of the DRB molecule, including pockets P1, P4, P7 and P9.

• 出版日期2012-11-30