Systemic cytokine response in moribund mice of streptococcal toxic shock syndrome model

作者：Saito Mitsumasa^*; Kajiwara Hideko; Iida Ken ichiro; Hoshina Takayuki; Kusuhara Koichi; Hara Toshiro; Yoshida Shin ichi

来源：Microbial Pathogenesis, 2011, 50(2): 109-113.

DOI：10.1016/j.micpath.2010.12.001

• Streptococcus pyogenes
• Streptococcal toxic shock syndrome
• Cytokine
• Mouse model

Streptococcus pyogenes causes severe invasive disease in humans, including streptococcal toxic shock syndrome (STSS). We previously reported a mouse model that is similar to human STSS. When mice were infected intramuscularly with 10(7) CFU of S. pyogenes, all of them survived acute phase of infection. After 20 or more days of infection, a number of them died suddenly accompanied by S. pyogenes bacteremia. We call this phenomenon "delayed death". We analyzed the serum cytokine levels of mice with delayed death, and compared them with those of mice who died in the acute phase of intravenous S. pyogenes infection. The serum levels of TNF-alpha and IFN-gamma in mice of delayed death were more than 100 times higher than those in acute death mice. IL-10 and IL-12, which were not detected in acute death, were also significantly higher in mice of delayed death. IL-6 and MCP-1 (CCL-2) were elevated in both groups of mice. It was noteworthy that not only pro-inflammatory cytokines but also anti-inflammatory cytokines were elevated in delayed death. We also found that intravenous TNF-alpha injection accelerated delayed death, suggesting that an increase of serum TNF-alpha induced S. pyogenes bacteremia in our mouse model.

• 出版日期2011-2