Adult olfactory sphere cells are a source of oligodendrocyte and Schwann cell progenitors

作者：Ohnishi Yu ichiro^*; Iwatsuki Koichi; Shinzawa Koei; Ishiharaa Masahiro; Moriwaki Takashi; Umegaki Masao; Kishima Haruhiko; Yoshimine Toshiki

来源：Stem Cell Research, 2013, 11(3): 1178-1190.

DOI：10.1016/j.scr.2013.08.005

The olfactory epithelial layer contains multipotent horizontal basal cells (HBCs) that differentiate into olfactory sensory neurons. Here, we show that rat HBCs express oligodendrocyte progenitor cell (OPC) and astrocyte markers. We generated olfactory sphere (OS) cells in cultures that were derived from adult rat olfactory mucosa. Fluorescence-activated cell sorting and-immunofluorescence analyses showed that OS cells also express OPC and astrocyte markers. Interestingly, OS cells underwent oligodendrocyte differentiation in vitro. To study oligodendrocyte differentiation in vivo, OS cells were transplanted into injured rat spinal cords. The transplanted cells integrated into host tissue and differentiated into oligodendrocytes. When transected saphenous nerve ends were encased in collagen-containing silicone tubes with or without OS cells, the transplanted OS cells differentiated into Schwann cells. Our data provide new insights into of the sternness of OS cells.

• 出版日期2013-11