Antitubercular constituents from Premna odorata Blanco

作者：Lirio Stephen B; Macabeo Allan Patrick G; Paragas Erickson M; Knorn Matthias; Kohls Paul; Franzblau Scott G; Wang Yuehong; Aguinaldo Ma Alicia M^*

来源：Journal of Ethnopharmacology, 2014, 154(2): 471-474.

DOI：10.1016/j.jep.2014.04.015

• Premna odorata
• Antitubercular
• Tuberculosis
• 1-heneicosyl formate
• Diosmetin

Ethnopharmacological relevance: Premna odorata Blanco (Lamiaceae) is a medicinal plant traditionally used in Albay Province, in southeastern Luzon, Philippines to treat tuberculosis. This study aimed to determine the antitubercular property of the crude extract and sub-extracts of the leaves, and to isolate the bioactive principles from the active fractions. %26lt;br%26gt;Materials and methods: Through extraction, solvent polarity-based fractionation and silica gel chromatography purification of the DCM sub-extract, compound mixtures from the bioactive fractions were isolated and screened for their in vitro antimycobacterial activity against Mycobacterium tuberculosis H(37)Rv using the colorimetric Microplate Alamar Blue assay (MABA). %26lt;br%26gt;Results: The crude methanolic extract and sub-extracts showed poor inhibitory activity against Mycobacterium tuberculosis H(37)Rv (MIC %26gt;= 128 mu g/mL). However, increased inhibitory potency was observed for fractions eluted from the DCM sub-extract (MIC=54 to 120 mu g/mL). Further purification of the most active fraction (MIC=54 mu g/mL) led to the isolation of a 1-heneicosyl formate (1), 4:1 mixture of p-sitosterol (2), stigmasterol (3) and diosmetin (4), which were identified through GC MS analysis (with dereplication) and NMR experiments. The MIC of compound 1 was 8 mu g/mL. %26lt;br%26gt;Conclusions: The results of this study provide scientific basis for the traditional use of Premna odorata as treatment for tuberculosis.

• 出版日期2014-6-11