科研之友
微信
新浪微博
Facebook
分享链接
摘要
A novel simple, sensitive fluorescence immunosensing method based on aptamer-plasmid complex amplification was developed. This method utilized the specific recognition between antibody and antigen as well as aptamer-plasmid complex and the intercalation of fluorescence dye SYBR Green I in the groove of duplex plasmid DNA in detection of Platelet-Derived Growth Factor BB (PDGF-BB). The immunoassay was performed in the microliter wells in which rabbit anti PDGF-BB antibody was immobilized. The PDGF 1313 analyte was captured by the primary antibody and then sandwiched by the aptamer-plasmid DNA complex. The introduction of fluorescence dye SYBR Green I allows for the detection of the sandwiched immunocomplex of antibody/anigen/aptamer-plasmid complex. Under the optimized conditions of salt concentration, ratio of aptamer to PUC19, and SYBR Green I concentration, the proposed method offers a linear detection range from 0.2 mu g/L to 200 mu g/L with a detection limit of 0.1 mu g/L.
- 出版日期2009-11
- 单位化学生物传感与计量学国家重点实验室; 湖南大学
