Molecular characterization of the herpes simplex virus 1 (HSV-1) homologues, UL25 to UL30, in duck enteritis virus (DEV)

作者：Liu Shengwang; Chen Shuhong; Li Huixin; Kong Xiangang^*

来源：Gene, 2007, 401(1-2): 88-96.

DOI：10.1016/j.gene.2007.06.022

• duck enteritis virus (DEV)
• gene arrangement
• UL25
• UL26
• UL26.5
• UL27
• UL28
• UL29 and UL30 gene
• molecular characteristics

A 16.6-kilo-base pair (kb) sequence was amplified from the duck enteritis virus (DEV) clone-03 strain genome using 'targeted gene walking polymerase chain reaction (PCR)'. Seven complete open reading frames (ORFs) were predicted, and designated herpes simplex virus 1 (HSV-1) homologues, unique long (UL) 25, UL26, UL26.5, UL27, UL28, UL29, and UL30. Sequence analysis revealed that the arrangement of seven genes in DEV clone-03 strain was collinear to that from HSV-1. In addition, mRNA transcription orientation was identical to the HSV-1 genes. While UL25, UL26, and UL26.5 shared the same poly A signal, the UL27 and UL28 genes overlapped by 211 bp nucleotides and shared the same 3' transcription terminus. UL26.5, an in-frame ORF of UL26, was co-terminal with UL26 at its 3'-end. We predicted that the gene arrangement in the unique long segment of the DEV clone-03 was identical to that in HSV-1, particularly in the region from UL25 to UL30 gene. Phylogenetic trees of the putative proteins encoded by these seven genes showed that UL27, UL28, and UL30 had a close evolutionary relationship with the Mardivirus. however, the other four proteins exhibited close relationships with the Simplexvirus or Varicellovirus, indicating that the DEV clone-03 should be placed into a single cluster within the subfamily Alphaherpesvirinae.

• 出版日期2007-10-15
• 单位中国农业科学院哈尔滨兽医研究所