Apoptotic Effects of Cordycepin Through the Extrinsic Pathway and p38 MAPK Activation in Human Glioblastoma U87MG Cells

作者：Baik Ji Sue; Mun Seo Won; Kim Kyoung Sook; Park Shin Ji; Yoon Hyun Kyoung; Kim Dong Hyun; Park Min Kyu; Kim Cheorl Ho; Lee Young Choon^*

来源：Journal of Microbiology and Biotechnology, 2016, 26(2): 309-314.

DOI：10.4014/jmb.1507.07090

• Cordycepin
• apoptosis
• extrinsic pathway
• U87MG cell
• p38 MAPK

We first demonstrated that cordycepin inhibited cell growth and triggered apoptosis in U87MG cells with wild-type p53, but not in T98G cells with mutant-type p53. Western blot data revealed that the levels of procaspase-8, -3, and Bcl-2 were downregulated in cordycepin-treated U87MG cells, whereas the levels of Fas, FasL, Bak, cleaved caspase-3, -8, and cleaved PARP were upregulated, indicating that cordycepin induces apoptosis by activating the death receptor-mediated pathway in U87MG cells. Cordycepin-induced apoptosis could be suppressed by only SB203580, a p38 MAPK-specific inhibitor. These results suggest that cordycepin triggered apoptosis in U87MG cells through p38 MAPK activation and inhibition of the Akt survival pathway.

• 出版日期2016-2