A dual functional fluorescent probe for glioma imaging mediated by Blood-brain barrier penetration and glioma cell targeting

作者：Ma, Hongwei; Gao, Zhiyong; Yu, Panfeng; Shen, Shun; Liu, Yongmei^*; Xu, Bainan

来源：Biochemical and Biophysical Research Communications, 2014, 449(1): 44-48.

DOI：10.1016/j.bbrc.2014.04.148

• AS1411 aptamer
• TGN peptide
• Glioma
• Non-invasive imaging

Glioma is a huge threat for human being because it was hard to be completely removed owing to both the infiltrating growth of glioma cells and integrity of blood brain barrier. Thus effectively imaging the glioma cells may pave a way for surgical removing of glioma. In this study, a fluorescent probe, Cy3, was anchored onto the terminal of AS1411, a glioma cell targeting aptamer, and then TGN, a BBB targeting peptide, was conjugated with Cy3-AS1411 through a PEG linker. The production, named AsT, was characterized by gel electrophoresis, H-1 NMR and FTIR. In vitro cellular uptake and glioma spheroid uptake demonstrated the AsT could not only be uptaken by both glioma and endothelial cells, but also penetrate through endothelial cell monolayer and uptake by glioma spheroids. In vivo, AsT could effectively target to glioma with high intensity. In conclusion, AsT could be used as an effective glioma imaging probe.

• 出版日期2014-6-20
• 单位中国人民解放军总医院; 中国人民解放军空军总医院; 四川大学; 复旦大学