Antigen-specific human NKT cells from tuberculosis patients produce IL-21 to help B cells for the production of immunoglobulins

作者：Wu, Changyou^*; Li, Zitao; Fu, Xiaoying; Yu, Sifei; Lao, Suihua; Yang, Binyan

来源：Oncotarget, 2015, 6(30): 28633-28645.

DOI：10.18632/oncotarget.5764

• NKT cells
• tuberculosis
• IL-21
• B cells
• immunoglobulins
• Immunology and Microbiology Section
• Immune response
• Immunity

Natural killer T (NKT) cells from mouse and human play an important role in the immune responses against Mycobacterium tuberculosis. However, the function of CD3(+)TCRv beta 11(+) NKT cells at the local site of M. tuberculosis infection remains poorly defined. In the present study, we found that after stimulation with M. tuberculosis antigens, NKT cells isolated from tuberculosis (TB) pleural fluid mononuclear cells (PFMCs) produced IL-21 and other cytokines including IFN-gamma, TNF-alpha, IL-2 and IL-17. IL-21-expressing NKT cells in PFMCs displayed effector memory phenotype, expressing CD45RO(high)CD62L(low)CCR7(low). Moreover, NKT cells expressed high levels of CXCR5 and all of IL-21-expressing NKT cells co-expressed CXCR5. The frequency of BCL-6-expression was higher in IL-21-expressing but not in non-IL-21-expressing CD3(+) TCRv beta 11(+) NKT cells. Sorted CD3(+) TCRv beta 11(+) NKT cells from PFMCs produced IFN-gamma and IL-21 after stimulation, which expressed CD40L. Importantly, CD3(+) TCRv beta 11(+) NKT cells provided help to B cells for the production of IgG and IgA. Taken together, our data demonstrate that CD3(+) TCRv beta 11(+) NKT cells from a local site of M. tuberculosis infection produce IL-21, express CXCR5 and CD40L, help B cells to secrete IgG and IgA, and may participate in local immune responses against M. tuberculosis infection.

• 出版日期2015-10-6
• 单位中山大学; 广州市胸科医院