Promoter Polymorphisms and Methylation of E-Cadherin (CDH1) and KIT in Gastric Cancer Patients from Northern Brazil

作者：Borges Barbara Do Nascimento^*; Santos Erivelton Da Silva; Matos Carvalho Bastos Carlos Eduardo; Pinto Laine Celestino; Anselmo Nilson Praia; Simoes Quaresma Juarez Antonio; Calcagno Danielle Queiroz; Rodriguez Burbano Rommel Mario; Harada Maria Lucia

来源：Anticancer Research, 2010, 30(6): 2225-2233.

• E-Cadherin
• promoter polymorphism
• methylation
• KIT
• CDH1
• gastric cancer

The aim of this study was to verify genetic and epigenetic alterations in gastric cancer patients from Para state, northern Brazil. Materials and Methods: Exon 11 of KIT and two promoter polymorphisms (-160 C/A and -347 G/GA) of the E-cadherin gene (CDH1), and their correlation with the promoter methylation status were analyzed. Results: No genetic alterations in KIT were found. Promoter polymorphisms revealed an increased probability of developing gastric cancer, especially of the diffuse-type, in patients carrying -160 A and -347 GA alleles. Analyses of CDH1 methylation suggested a significant difference between hypermethylated and non-hypermethylated samples, with a positive association between the -160 A allele and hypermethylation. Conclusion: Our results suggest that -160 A and -347 GA polymorphisms may increase the chance of developing gastric cancer in the studied population and that -160 A polymorphism seems to be related to the hypermethylation pattern of the promoter region of CDH1.

• 出版日期2010-6